MALDI mass spectrometry imaging (MSI) is a powerful technology that is being used to visualize the spatial distribution of dosed drugs, metabolites, lipids, peptides and proteins directly from the surface of a tissue section. The AB SCIEX TOF/TOF™ 5800 System is the fastest, most sensitive, and most flexible platform for MALDI MSI of tissue sections.
Highest sensitivity and speed
- Fastest MSI system available due to improveme
nts to the laser and a continuous motion sample plate.
- Flexiblility for high-speed, low-resolution survey images and high-definition spatial resolution of down to 1µm on the same tissue sample.
- High sensitivity Mass Spectrometry (MS) and MS/MS data on a wide range of molecules from the same tissue section, including small molecules, peptides, and proteins.
- A new self-cleaning MALDI source overcomes the drawbacks of system contamination from tissue samples to significantly increase system up-time and productivity.
Flexibility of Imaging Applications
Anlayze the spatial distribution of dosed drugs, metabolites, lipids, peptides, and proteins directly from the surface of a tissue section. The AB SCIEX mass spectrometry tissue imaging workflow using the AB SCIEX TOF/TOFTM 5800 System and the 4800 Plus MALDI TOF/TOFTM Analyzer provide the fastest, most sensitive, and most flexible platforms for MALDI mass spectrometry imaging.
Fig 1: Profiling of Intact Protein from Rat Brain Tissue by MALDI MSI. (a): complete brain images. (b): expanded regions.
(a)
+ View Larger
(b)
+ View Larger
|
Fig 2: Profiling of Peptide from Rat Brain Tissue by MALDI MSI
+ View Larger
|
Fig 3: Profiling of Lipids from Rat Brain Tissue by MALDI MSI
+ View Larger
|
Fig 4: Identification of Lipids from Rat Brain Tissue by MS/MS MALDI MSI
+ View Larger
|
Fig 5: Identification of Neuropeptides from Rat Brain Tissue by MS/MS MALDI MSI
+ View Larger
|
|
|
AB SCIEX TOF/TOFTM 5800 System
The AB SCIEX TOF/TOFTM 5800 System provides the most sensitive and confident path for MALDI mass spectrometry imaging. It offers supperior MS/MS capabilities for the most confident identifications of peptides and lipids directly from tissue.
- More than 10X faster data acquisition with the new 1KHz laser and DynamicExitTM algorithm.
- Unique OptiBeamTM on-axis laser irradiation increases sensitivity in both MS and MS/MS modes by 10x over systems without an on-axis laser.
- Maximum up-time and throughput by continuous heating of the source mirror and user programmable source mirror cleaning.
- Improved resolution with new reflectron mirror design and 1000 MHz digitizer.
- High-resolution precursor ion selection with novel timed-ion selector.
|
|
4800 Plus MALDI TOF/TOFTM Analyzer
The 4800 Plus MALDI TOF/TOFTM Analyzer provides high sensitivity and mass range for confident MALDI mass spectrometry imaging of a wide range of analytes.
- Ideal system for protein biomarker discovery, providing simultaneous identification and quantitation of up to 8 samples at a time (8-plex workflow) using iTRAQ® Reagents.
- OptiBeamTM on-axis laser provides ten times greater sensitivity to identify and quantitate low-abundance proteins in complex biological samples.
- High-resolution precursor ion selection, enabled by the QuanTISTM precursor selector, lets you detect and identify peptides that other systems miss and makes it easier for you to interpret your data by simplifying MS/MS spectra.
- High-energy, information-rich fragmentation patterns unavailable on other instrument platforms enable you to analyze carbohydrates, lipids, and other biological components.
|
|
One combination of offerings to help you perform MALDI mass spectrometry imaging analysis is below:
Instruments
|
|
AB SCIEX 5800 TOF/TOFTM System: The AB SCIEX 5800 TOF/TOFTM System has the highest sensitivity and speed for MSI Imaging, plus increased robustness for maximum productivity. |
|
|
AB SCIEX 4800 Plus MALDI TOF/TOFTM Analyzer: The high sensitivity and mass range of the AB SCIEX 4800 Plus TOF/TOFTM Analyzer combine to provide high quality MS and MS/MS data for identification and localization of analytes for MALDI mass spectrometry imaging. |
Software
|
 |
TissueViewTM Software: TissueViewTM Software rapidly display the spatial location and intensity of drugs, metabolites, lipids, peptides and proteins in all tissue types and supports all AB SCIEX MALDI TOF/TOFTM platforms. It provides a seamless link between optical images, MS images, MS and MS/MS spectra, with software for processing and displaying mass spectrometric imaging (MSI) data (Analyze 7.5). |
|
 |
Mass Spectrometry Imaging Data Acquisition Tool:This software was specifically written for image acquisition on AB SCIEX 4000 Series MALDI TOF/TOFTM Instruments. It integrates seamlessly with the 4000 Series Explorer software and allows the acquisition of images in MS/MS mode. This software is available fromhttp://www.maldi-msi.org. |
Accessories
|
 |
HTX Technologies TMTM Sprayer: The HTX Technologies TMTM Sprayer provides high efficiency matrix/analyte co-crystallization for superior MALDI ion signal. The patented capillary nozzle matrix deposition system and intuitive operator interface enable creation of custom spray patterns and sequences, all with high throughput (~ 12 min/plate). |
|
 |
LaserBio LabsTM Mass Spectrometry Imaging Starter Kit: The MSI Starter Kit from LaserBio LabsTM includes a plate adaptor for two microscopic glass slides, ITO coated conductive glass slides, double sided adhesives and copper tape, calibrants and matrix, and water-sensitive papers for optimization of robotic spray conditions. |
|
View brochures, technical notes, webinars and videos about how we can help you image drugs, metabolites, lipids, peptides and proteins.
Media
Brochures
Technical Notes
Videos
|
