Tissue Imaging

Visualize the spatial distribution of dosed drugs, metabolites, lipids, peptides, and proteins directly from the surface of a tissue section

For research use only. Not for use in diagnostic procedures.

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a powerful technology for probing and analyzing the spatial arrangement of molecules directly in thin tissue sections. Tissue imaging mass spectrometry can be used to determine the distribution of hundreds or even thousands of unknown analytes such as proteins, peptides, lipids, drugs, or metabolites in a single experiment. Ultimately, mass spectrometry imaging will help deepen our understanding of the molecular mechanisms underlying normal biological processes and human disease.

Applications for mass spectrometry imaging include profiling of endogenous biomolecules within tissues/organs, comparisons of analyte abundances between samples taken at different times or after different treatments, and analysis of the distribution and metabolism of dosed drugs.

AB SCIEX is a global leader in developing tools for mass spectrometry imaging. Our instrumentation, software, reagents, and workflows deliver the accuracy, precision, and reproducibility required for even the most challenging surface and tissue imaging experiments.

The AB SCIEX TOF/TOF™ 5800 System is the world's most sensitive MALDI TOF/TOF system for mass spectrometry imaging. It can identify and characterize biomolecules in applications ranging from tissue mapping to proteomic discovery – 10-fold faster and with 10-fold more sensitivity than any other MALDI TOF/TOF instrument. For mass spectrometry imaging, the TOF/TOF™ 5800 is the only system capable of acquiring MALDI MS/MS data from tissue samples, due to its unique QuanTIS™ Precursor Ion Selector and OptiBeam™ On-axis Laser. With its TOF/TOF™ Imaging Acquisition Software, the TOF/TOF™ 5800 System provides a complete, industry-leading solution for tissue imaging with micron-spatial resolution of larger biomolecules.

Highest sensitivity and speed

  • Fastest MSI system available due to improvemeImaging of lipids from rat brainnts to the laser and a continuous motion sample plate
  • Flexibility for high-speed, low-resolution survey images and high-definition spatial resolution of down to 1 µm on the same tissue sample
  • High-sensitivity mass spectrometry (MS) and MS/MS data on a wide range of molecules from the same tissue section, including small molecules, peptides, and proteins
  • A new self-cleaning MALDI source overcomes the drawbacks of system contamination from tissue samples to significantly increase system up-time and productivity

With the new Advion LESA® Clarity system, the AB SCIEX QTRAP® 5500 and TripleTOF™ 5600+ Systems now complement the TOF/TOF™ 5800 System for mass spectrometry imaging for drugs, metabolites, and other fragile analytes. LESA® Clarity brings the benefits of nanoelectrospray/mass spectrometry to surface analysis and automates surface sampling for faster, more effective analysis. LESA® Clarity enables direct screening off of any surface, without the need for sample preparation.

TissueView™ Software rapidly displays the spatial location and intensity of drugs, metabolites, lipids, peptides, and proteins in all tissue types and supports all AB SCIEX MALDI TOF/TOF™ Platforms. It provides a seamless link between optical images, MS images, MS and MS/MS spectra, with software for processing and displaying mass spectrometric imaging data.

 

    Flexibility of imaging applications

    Analyze the spatial distribution of dosed drugs, metabolites, lipids, peptides, and proteins directly from the surface of a tissue section. The AB SCIEX mass spectrometry tissue imaging workflow using the AB SCIEX TOF/TOF™ 5800 System and the 4800 Plus MALDI TOF/TOF™ Analyzer provide the fastest, most sensitive, and most flexible platforms for MALDI mass spectrometry imaging.


    Fig 1: Profiling of intact protein from rat brain tissue was achieved by MALDI-MSI: (a) complete brain images, and (b) expanded regions.

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    Fig 2: Profiling of peptides from rat brain tissue was achieved by MALDI-MSI.



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    Fig 3: Profiling of lipids from rat brain tissue was acheived by MALDI-MSI.



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    Fig 4: Identification of lipids from rat brain tissue was achieved by MS/MS/MALDI-MSI.



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    Fig 5: Identification of neuropeptides from rat brain tissue was achieved by MS/MS/MALDI-MSI.



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