Vitamin D deficiency has been linked to various cancers, autoimmune disorders, heart disease, diabetes, and other health problems. This has led to a significant increase in the research involving Vitamin D analysis over the past two years. As a result, LC/MS/MS has quickly become the technique of choice for research involving the detection and quantification of Vitamin D in biological samples.
The routine adoption of liquid chromatography-tandem mass spectrometry (LC/MS/MS) for clinical research is rapidly gaining momentum. For challenging research applications such as the quantitative measurement of low-level steroids, immunoassay-based technologies have demonstrated insufficient specificity, resulting in poor accuracy and precision. Therefore, there are considerable advantages to be gained by switching to LC/MS/MS as the research technique of choice.
Figure 1: Example chromatogram of a control sample for OH-Vitamin-D3 and OH-Vitamin-D2 with overlaid MRM ion traces for the quantifier and qualifier MRM transitions performed on a 3200 QTRAP® System in a 4 minute run.
The AB SCIEX Triple QuadTM 5500 system is today's most sensitive triple quadrupole mass spectrometer that is designed to deliver high levels of sensitivity and robustness for even the most complex and demanding matrices. The AB SCIEX Triple QuadTM 5500 exceeds the performance required by even the most demanding clinical research studies and excels at multi-component quantitation required by endocrinology and biomarker research applications such as dihydroxy-vitamin D2 and D3 analysis
Robust, high-throughput platform for all demanding applications and matrices
Excel at highly multiplexed peptide quantitation
Unequalled accuracy and precision for quantitative analysis
Smaller footprint
Lowest limits of detection available for clinical research studies
One combination of offerings to help you analyze Vitamin D is below:
Instruments
3200 QTRAP® LC/MS/MS System: Provides excellent sensitivity, superior dynamic range, and reliability across a range of identification and quantitation applications including research involving monohydroxy-vitamin D2 and D3 analysis.
AB SCIEX QTRAP® 5500 LC/MS/MS system: the next-generation technology which is designed to excel at metabolite identification, detection and confirmation of low-level compound quantitation for biomarker verification and validation research.
Software
Cliquid® 3.0 Software: Designed to work in conjunction with Analyst® Software to provide a simple four step research workflow for routine analysis by LC/MS/MS to simplify set-up and analysis, from sample processing to reports.
iMethodTM Tests
Pre-configured methods for research involving the analysis of vitamin D, which will save you method development time and costs when implementing LC-MS/MS in your research laboratory.
View brochures, technical notes, webinars and videos about how we can help researchers analyze Vitamin D.
