Because of the importance of amino acids in metabolic and cellular functions, biological samples have been analyzed extensively in research to quantify amino acids associated with various metabolic disorders.

Faster Time to Result

AB SCIEX LC/MS/MS amino acid analysis solution for researchers is faster than conventional research methods and uses labeled internal standards for more accurate and robust quantitation. Specially designed Cliquid® Software provides research workflow, methods, and report templates for relative and absolute amino acid quantitation of 45 amino acids from protein hydrolysate samples. Researchers can nearly double their throughput, using the Cliquid® MPX^TM-2 High Throughput System to control and perform staggered injections from two HPLC systems.

Fig. 1: Chromatogram of the separation of a mixture of amino acid standards. The amount of each amino acid injected on the column was 10 pmole (except 5 pmole for cystine). Detection was performed using the 3200 QTRAP® system

API 3200™ LC/MS/MS System

The API 3200™ LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package.

• Innovative Turbo V™ ion source efficiently ionizes compounds over a wide range of flow rates, virtually eliminating cross-contamination, even with large sample loads
• Patented LINAC® collision cell technology delivers superior MS/MS performance allowing faster scan times without compromising sensitivity or mass spectral quality
• Simplifies cross-platform methods transfer
• Gives meaningful results from every experiment

Our LC/MS/MS amino acid analysis solution for researchers is faster than conventional methods and uses labeled internal standards for more accurate and robust quantitation.:

Instruments

AB SCIEX Triple Quad™ 5500 LC/MS/MS system: Today's most sensitive triple quadrupole mass spectrometer that is designed to deliver high levels of sensitivity and robustness for even the most complex and demanding matrices used in research. The AB SCIEX Triple Quad™ 5500 exceeds the performance required by even the most demanding clinical research studies and excels at multi-component quantitation.

Software

		Cliquid® 3.0 Software: Designed to work in conjunction with Analyst® Software to provide a simple four step research workflow for routine analysis by LC/MS/MS to simplify set-up and analysis, from sample processing to reports.
		Designed specifically for clinical researchers, our ChemoViewTM Software 2.0 automatically measures the response of various compounds and calculates concentrations and equations of concentrations in large sets of data, highlighting results that fall outside limits set up by the researcher. ChemoViewTM Software, combined with industry leading triple quadrupole systems, front-end HPLC, and iMethodTM Tests, provides a research solution that is quick to implement and delivers confident, cost-effective analyses.

iMethods

iMethod™ Test for aTRAQ™ 1.0 including Cliquid AA 3.0 SW License: This provides a method for research requiring the analysis of 45 Amino Acids in physiological fluid using amino reactive isotope coded tags in conjunction with LC/MS/MS. The use of an internal standard adds quantitative speed and confidence in research results. The total analytical run time is less than 25 minutes.

View brochures, technical notes, webinars and videos about how we can help researchers perform amino acid analysis.

Media

Brochure

• Brochure: High performance, higher productivity, greater value: API 3200^TM LC/MS/MS System