In Vivo PK & Metabolite ID

Traditionally, in late stage discovery, the quantitative bioanalysis is performed on samples from PK studies while the in vivo metabolite identification is done in a second separate analysis and often on two different types of mass spec instruments.  In order to provide a significant enhancement in speed and efficiency it becomes highly beneficial to conduct both the in vivo PK quantification and identification of the major metabolites on a single instrument platform. 

The TripleTOF^TM5600 has the speed, accuracy and resolution in both MS and MS/MS modes to enable simultaneous quantitation and metabolite identification from in vivo PK studies at therapeutics does levels.  The unique ability to conduct real time multiple mass defect filtering on a chromatographic time scale means there is no need for a 2nd injection to acquire quality MS/MS data.  MetabolitePilot^TM Software facilitates the data processing and interrogation while MultiQuant^TM Software can obtain the HRMS PK profiles of the parent and relative quanitification of metabolites in an efficient and simple manner.

 With the AB SCIEX TripleTOF^TM 5600 System it is possible to simultaneously conduct both quantitative PK analysis of the parent drug and its major metabolites, as well as the qualitative identification of the major metabolites, in a single analysis

• Single injection workflow for both quantitative and qualitative data acquisition
• Increase metabolite id workflow efficiency with real time multiple mass defect filtering for MS/MS
• Automated batch processing for metabolite id at different PK time points