Taking a SLICE out of Lipidomics Complexity: Differential Mobility Ion Selection and Lipids
Presented by: Dr. Paul Baker, AB SCIEX Sr. Applications Specialist (NA), Global Applications Lead in Lipidomics
Advances in mass spectrometry have enabled in-depth lipidomic analysis with unparalled quantitative sensitivity. However, unambiguous identification and quantitation of lipid molecular species in total lipid extracts has proven to be difficult. Greater than 100,000 lipid molecular species are present in a typical biological lipid extract; these occupy a narrow mass range (~400-1100 amu), making isobaric overlap a significant problem. Currently, this problem is addressed by extensive and sometimes complex chromatography, including fractionation, 2-D LC strategies, and chiral chromatographic columns. SelexION™ Differential Mobility Separation Technology provides an alternative to these complicated chromatographic methods, allowing molecules to be separated based on differential ion mobility prior to mass spectrometry analysis.
In this presentation, we present data demonstrating SelexION™ Technology effectively resolves multiple lipid classes from complex mixtures prior to analysis by mass spectrometry. By removing isobaric interference, lipid analysis by QTRAP® Systems or TripleTOF® Systems using SelexION™ technology enables unambiguous identification of lipid molecular species and provides for more accurate relative quantitation by MS/MS.
Who should attend?
All scientists doing lipids, lipidomics, and metabolomics analysis.
15:00 (London, BST, UTC +1 hour)
16:00 (Paris, CEST, UTC +2 hours),
10:00 (New-York, EDT, UTC -4 hours)
Duration: 60 minutes (including chat Q&A session)
Check your local time zone
|Dr. Paul Baker
AB SCIEX, Sr. Applications Specialist (NA), Global Applications Lead in Lipidomics
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|July 16, 2013 - July 16, 2013|