SelexION™ Technology

SelexION™ Technology brings unprecedented analytical selectivity enhancements and data quality improvements for your most challenging assays.
SelexION™ Technology brings unprecedented analytical selectivity enhancements and data quality improvements for your most challenging assays.
SelexION™ Technology turns the worlds most sensitive triple quadrupole and QTRAP into the world's most sensitive and selective triple quadrupole and QTRAP.
Bring a new dimension of selectivity, simplicity and cost effective efficiency to your LC/MS/MS analysis.
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The AB SCIEX SelexION™ Technology device is an effective ion mobility spectrometry tool for improving data quality in the quantitation and characterization of challenging samples requiring advanced analytical selectivity.

SelexION™ Technology on the AB SCIEX Triple QuadTM 5500 and QTRAP® 5500 Systems delivers a new dimension of selectivity and performance for applications requiring the separation of isobaric species, elimination of challenging co-eluting contaminants and reduction of high background noise.

  • Adds differential ion mobility spectrometry to AB SCIEX Triple Quad™ 5500 and QTRAP® 5500 Systems with a compact, easily interchangeable unit.
  • 25ms cycle time per MRM- matches cycle and run times with multi-component analysis and UHPLC time scales
  • Easy to mount/dismount - can be setup in less than 2 minutes without the need to break vacuum and does not require any tools.
  • Integrated Chemical Modifier - ability to add a chemical modifer to increase peak separation power when needed.
  • Stability and reproducibility - proven for regulated bioanalysis guidelines.

 

Status: Available Item status information

 

The AB SCIEX SelexION™ Technology device is an innovative achievement in differential ion mobility spectrometry. The SelexION ion mobility spectrometry tool has the reproducibility, robustness and ease of use to deliver highly selective  quantitative and qualitative analyses, is compatible with UHPLC  time scale and over multiple MRM's simultaneously - all on the worlds most sensitive triple quadrupole and QTRAP platforms.

For scientists who are challenged by assays with isobaric interferences and difficult to separate co-eluting contaminants and who require a fast, reproducible and easy to use approach to enhance the selectivity of their  LC/MS/MS separations.

SelexIONTM takes the worlds most sensitive Triple QuadTM and QTRAP® Systems and adds a new Dimension of Selectivity for enhanced quantitative and qualitative performance.

Feature Benefit
Scan Speed The device can acquire data for a single MRM transition in 25 msec including the inter-scan pause time of 20 msec.  4X faster than cylindrical ion mobility.  Amenable to multiple analyte analysis and UHPLC time scales.
Tuning Stability The optimum Compensation Voltage (COV) does not shift by more than 10% of the DMS peak width (full width at half height, typically 2.5V) at a fixed Separation Voltage (SV) over 24 hours on an equilibrated system operating under standard DMS resolution gas setting. Suitable for quantitative/regulated lab applications.
Integrated Chemical Modifier Can increases the ion mobility separation power by up to 10X.
Mounting and dis-mounting User mountable and dis-mountable without the need for tools or breaking vacuum.  Compact design takes <2 mins to install or remove when at room temperature.  Ideal for quick deployment during method development process.
Scheduled MRMTM Algorithm Support Compatible with the Scheduled MRMTM Algorithm which provides automatic, dynamic adjustment of the acquiring MRM transitions such that only compounds that elute within a specified time window are monitored allowing the system to dynamically schedule MRM scans during the experiment.
Reproducibility & Robustness SelexION: Reproducibility and Robustness Proven to meet regulated bioanalysis guidelines.
A Clear Difference SelexION: A Clear Difference Injection of clenbuterol at 5 pg/µL in urine: (a) High chemical noise makes the detection impossible. (b) Differential ion mobility spectrometry with SelexION™ technology increases selectivity and eliminates the high background producing a 10X improvement in signal-to-noise.
Background-Checked SelexION: A Clear Difference Stable isotope labeled synthetic peptides generated for quantitation of a therapeutic IgG in digested human plasma showed much cleaner MRM signals at the same on-column amount when an ion mobility separation was performed.  This yielded a 5X improvement in the LLOQ achieved on column.
Above the Noise         SelexION: A Clear Difference Significantly reduce and eliminate isobaric intereferences and co-eluting contaminants.  Grape fruit extract fortified with conazole degradant products at 0.01 mg/kg.  Samples kindly supplied by R.Schoening, Bayer CropScience, Monheim, Germany and J.Jasak, Technical University, Institute of Frood Chemistry, Dresden, Germany.
Multiplied Selectivity    SelexION: A Clear Difference Improving quantitation by reducing strucally similar interferences.  A 30 µL injection of eicosanoids standard mix containing Prostaglandin F2a (PGF2A). (a) Interference with structurally similar species leads to difficulties in identification and quantitation.  (b) SelexION™ technology eliminates isobaric interferences and chemical background.