AB SCIEX | Mass Spec Products | Software | LipidView™ Software

LipidView™ Software

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LipidView^™ Software is a data processing tool for the molecular characterization and quantification of lipid species from electrospray MS data. The software enables lipid profiling by searching parent and fragment ion masses against a lipid fragment database containing over 25,000 entries, and reports a numerical and graphical output for various lipid molecular species, lipid classes, fatty acids and long chain bases*. LipidView^™ software streamlines a number of key steps such as automated data processing derived from template methods, method editing and selection, lipid species identification, comprehensive isotope contribution removal, multiple internal standards-based quantification, visualization and result reporting. Combined with the AB SCIEX QTRAP® 5500 System, or the AB SCIEX TripleTOF^™ 5600 System, this complete hardware and software package offers unique data acquisition strategies coupled with automated data processing for lipid profiling.

Trial Software Downloads
To download a trial version and obtain license keys, visit our LipidView™ Trial Software Download Registration Site

44 lipid classes, over 25,000 lipid species represented in a lipid fragments database
More than 600 characteristic lipid fragments listed
Lipid catalogue, lipid calculator utilities
Capabilities to process AB SCIEX Triple Quad^™, QTRAP®, TripleTOF^™ and QSTAR® system data
Support of lipid bioinformatics in medium sample throughput

Status: Available

Highlights

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The LipidView^TM software Catalogue Utility The LipidView™ software Catalogue Utility (Figure 1) includes over 25,000 possible lipid species and has information on parent formula, molecular mass, isotope distribution correction factor, charge states, adduct modification, and characteristic fragments for each. In addition, the LipidView^™ software Calculator utility (not shown) retrieves lipids that correspond to a given m/z and can filter out species with uncommon chain compositions.

Figure 1: LipidView^™ software Catalogue Utility showing data entry for PE 38:4

LipidView Software Catalogue Utility-figure1 showing data entry for PE 38:4

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Identify Lipids in 4 Steps LipidView^{^TM} software is structured around four simple steps as shown in the top left Workflow box in Figure 2. Initially, raw data are loaded into the software and then the processing settings are assigned. Different processing parameters can be set for each sample which facilitates the handling of sample batches. For each selected method the properties are displayed in the "Analysis method properties" window. After sample processing using the "Find Lipids" button, the data can be viewed in the Results workspace. Additionally user-defined reports can be generated.

Figure 2: Processing Setup Window in LipidView™ software showing separate methods per sample.

Processing Setup Window in LipidView Software-figure2

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Lipid Results Workspace The Lipid Results Workspace (Figure 3) has a Numerical Results View (top) and a Profile View (bottom). In the Numerical View, samples are listed for side-by-side comparisons and peak intensities, peak areas, corrected values, etc are displayed. All of the columns can be sorted, the samples can be filtered and grouped, and all of the data can be exported in a .txt format.

The basic Profile View directly links to the entries highlighted upper numerical table and can be expanded into an Advanced Profile view which enables sample grouping and the plotting of all lipids, class-specific, fatty acid data etc. The raw data can be retrieved in the Spectrum view (Figure 4) where lipid peaks can be labeled and spectra can be overlaid, summed and subtracted.

Figure 3: LipidView^™ software Results Workspace

LipidView software Results Workspace-figure3