Process MRM and Full Scan Data Quantitation MultiQuant™ Software is a powerful and easy-to-use quantitation package that processes MRM and full scan data for quantitation. New advanced features in the latest version 2.0 allow streamlined quantitation for many workflows including regulated and non-regulated quantitation, protein/peptide quantitative workflows, as well as powerful functionality for assays with hundreds or even thousands of analytes. The new advanced SignalFinder™ integration algorithm generates reliable integration results faster and with less user intervention than ever before, helping analysts maximize their productivity.
Intuitive User Interface In the MultiQuant™ Software user interface is organized so that important information is at your finger tips. The navigation window makes it easy for you to move from one analyte group to the next, to display results from internal standards or from analytes only, and to review specific transitions.
Figure 1: Easy-to-use Interface
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The ability to sort MRM data by quality or signal/noise ratio enables you to focus your review on only the lowest scoring MRM transitions. An overlay view of multiple MRM transitions for the same species allows you to assess peak shape to scan for possible peak interferences in selected channels. Many other ease-of-use features minimize mouse clicks and reduce the data review bottleneck in extracting quantitative information from every sample set. Using flexible user interface tools, you can even fine tune the software's display based on your specific data processing needs (see Figure 1).
Supports Relative and absolute Quantitation with Labeled or Unlabeled Internal Standards MultiQuant Software enables you to process large relative quantitation proteomics datasets. In relative quantitation experiments, you evaluate MRM data from multiple analytes across multiple samples and compare the relative amounts of analyte present in each sample. You can do this by either using raw MRM peak areas or labeled internal standards, such as mTRAQ™ reagent labeled samples, and then assessing MRM peak area ratios for more accurate calculations (see Figure 2). You can also perform absolute quantitation of analytes using MultiQuant™ Software.
Figure 2: Relative Quantitation Experiments
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In absolute quantitation experiments, you generate a standard concentration curve using internal standard peptides, process your biological samples (your unknowns), and then determine the amount of analyte present in each sample by comparing it to the standard curve (see Figure 3).
Figure 3: Chromatogram of Acidic Pesticides at 10ng/mL
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Innovative Audit Trail Functionality MultiQuant 2.0 introduces a new robust audit trail, with innovative features such as Grouped Audit Events and powerful audit trail management and review functionality. The new Audit Map Editor allowsthe laboratory administrator precise control over audit trail functionality using an intuitive interface. MultiQuant 2.0 also introduces dedicated data review functionality. The audit trail and the data are linked, and all versions of the data are stored. A reviewer can now view the data before and after an auditable change. Data review and audit trail view panes are linked for easy and efficient data review.
Figure 4: Powerful Audit Trail Viewer
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Quality Assurance MultiQuant 2.0 software has been designed to facilitate QA and supervisor review of the results, including review across multiple batches within a project. For example, all result tables in a given project can be scanned to find a specific event or action captured in the audit trail. In addition, the reviewer can track a single sample across multiple batches, easily locating the original injection and re-injections in different results tables. The raw data for each injection and the integration can be easily check and tracked.
Data Integrity Data integrity is assured throughout. The software allows locking of the data to prevent further changes. Locking/unlocking authority is defined by the administrator who can also require that data be locked prior to exporting to LIMS or printing. The software also requires the operator to save the results prior to printing or exporting. Finally, a dedicated data review function has been added to allow QA review without the potential of accidentally changing the data.
Takes advantage of the MIDAS™ Workflow The MIDAS Workflow combines a MRM scan with a full product ion scan, enabling you to confirm sequences easily by examining all fragment ions in the same spectrum. Since MRM is such a sensitive technique, the full scan MS/MS experiment must also be highly sensitive in order to acquire high quality MS/MS data from lower level precursors. QTRAP® systems combine triple quadrupole MRM selectivity with linear ion trap MS/MS sensitivity and are the only systems on the market to support the MIDAS™ Workflow with the sensitivity required in MS/MS mode. MultiQuant™ Software processes both MIDAS Workflow data as well as MRM-only data.
Allows exporting of Data In addition to being easy to use, MultiQuant™ Software allows you to visualize results within the software or easily export them for use in other software packages (e.g. Microsoft Excel). And using the software's flexible reporting features, it's easy to create your own customized reports.
For Research Use Only. Not for use in diagnostic procedures.
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